FLOW CYTOMETRY ANALYSIS

Owing to its precision, its rapidity and the multiple options that it offers to users, flow cytometry is still one the most popular immunological techniques. It uses fluorochrome-conjugated antibodies that specifically bind to their extracellular and intracellular antigens and thus offers accurate identification of multiple cell populations and the assessment of their activation status. Our cutting edge flow cytometry technology from Beckman Coulter allows the detection of up to 13 markers simultaneously with an excellent resolution of small particles up to 100 nm even in most complex biological fluids. The expertise of our scientists will provide you with step by step guidance and with customized protocols to help you catch the smallest details with the highest sensitivity in any type of sample.

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Immunophenotyping

IMMUNI T provides an array of panels for phenotyping various cell populations. All the available panels can be customized and new panels can be designed based upon your needs.
Pre-designed panels are available for the phenotyping of the following cell populations:

  • T lymphocytes: CD4 subsets (Treg, Th1, Th2, Th17), CD8 subsets

  • B lymphocytes: B cells, plasma cells, Breg

  • NK/NKT cells

  • Monocytes, granulocytes and dendritic cells

  • MDSC (Myeloid Derived Suppressor Cells)

Cell counts, enumeration, ratios of biomarkers of interest, percent expression to mother gate are determined for each panel analyzed. The extent of analysis and the form of presentation of the data are performed as desired.

Biomarker analysis

IMMUNI T provides various solutions for assessing the functional state of immune cells, establishing the desired profiles of cell populations as desired. Activation status, immune checkpoints, phosphorylation state of intracellular molecules, cytokine secretion with both intra- and extra-cellular measurements, cell degranulation and proliferation state can be assessed. These measurements lead the way to biomarker discovery/assessment in response to a particular treatment.

The following capabilities are offered:

  • Intracellular staining

    • Phosphorylated/unphosphorylated status of transcription factors or kinases

    • Expression level of intracellular cytokines and proteins

  • Peptide/protein detection and measurements

    • Multiplex cytokine measurements on CBA platform (Cytometric Bead Array)

    • Cell surface marker density

      • Cell identity

      • Activation status

  • Proliferation and viability

    • CFSE, CTV, eFluor670, Ki-67 staining

    • 7-AAD and other viability dyes